In this lesson the students will:Define the terms nucleotide, deoxyribose, phosphate group, nitrogenous base, antiparallel, and hydrogen bonding, purine, pyrimidine, and complementary base pairing.Identify the components of a nucleotide.Describe the structure of DNA.Create models of DNA using complementary base pairing.
Material Type: Lesson
In this lesson the students will:Define the terms initiation, elongation, termination, origin of replication, helicase, RNA polymerase, DNA polymerase III, okazaki fragments, DNA polymerase I, telomeres, semiconservative, leading strand, and lagging strand. Summarize the stages and processes related to DNA replication.
Material Type: Lesson
In this lesson the students will:Construct a model of the structure of DNA.Identify errors in a DNA sequence.
Material Type: Lesson
In this lesson the students will:Define the terms polymer, ribose sugar, central dogma of molecular biology, transcription, and RNA polymerase. Describe the similarities and differences between DNA and RNA structure.Utilize the process of transcription to create DNA models.
Material Type: Lesson
In this lesson the students will:Define the term genetically modified organism.Demonstrate ability to follow protocols.Isolate DNA for later application and analysis.
Material Type: Activity/Lab, Lesson
In this lesson the students will:Define terminology.Describe DNA sequencing methods, such as Sanger and Next Generation Sequencing.Compare and contrast different DNA sequencing approaches to provide recommendations for which approach works best for given scenarios.
Material Type: Lesson
In this lesson the students will:Define electrophoresis and DNA fingerprint.Distinguish between vertical and horizontal gel electrophoresis.Explain the relationship between agarose concentration and pore space.
Material Type: Lesson
In this lesson the students will:Calculate % agarose and the mass of powdered agarose needed for a specific agarose concentration.Prepare an agarose gel following a SOP for a future use in analysis. Practice loading sample dye into an agarose gel in buffer solution.
Material Type: Lesson
In this lesson the students will:Define the term electrolysis.Make 1X TAE solution for use in gel electrophoresis.Identify common gel electrophoresis errors.Conduct a gel electrophoresis and interpret the results.
Material Type: Activity/Lab
In this lesson the students will:Define the terms restriction enzymes, restriction site, sticky end, blunt end, restriction fragments, and DNA fingerprinting.Explain how restriction enzymes function.Examine the use of restriction enzymes in DNA fingerprinting.Model how restriction enzymes function using a simulation.
Material Type: Lesson
In this lesson the students will:Define lambda DNA and reaction buffer.Evaluate the reaction conditions necessary for restriction enzymes.Perform DNA digestion with restriction enzymes on lambda DNA.
Material Type: Activity/Lab
In this lesson the students will:Define DNA stain and loading dye. Prepare 0.8% agarose gels for gel electrophoresis.Conduct gel electrophoresis to separate DNA fragments from restriction enzyme digestion. Explain how DNA stains help to visualize DNA fragments.
Material Type: Activity/Lab
In this lesson the students will:Define the terms ribosome, ribosomal RNA, transfer RNA, amino acid, peptide bond, protein, translation, and codon.Describe the process of translation of DNA in order to build proteins.Utilize a codon table to model the process of translation to create protein molecules.
Material Type: Lesson
In this lesson the students will:Define semi-log graph.Construct standard curve for HindIII cut DNA using semi-log graph paper.Explain the relationship between number of base pairs and distance migrated.Calculate the size of the DNA fragments cut with EcoRI based on a standard curve.
Material Type: Activity/Lab
In this lesson the students will:Define polymorphism, restriction fragment length polymorphism (RFLP), non-coding DNA, variable number tandem repeats (VNTR), chromosomal locus, and Combined DNA Index System (CODIS). Explain the role of VNTRs in RFLP.Identify uses of VNTR analysis.Examine how VTNRs are used in DNA fingerprinting.
Material Type: Lesson
In this lesson the students will:Define Southern blot, DNA probe, DNA denaturation, hybridization, and autorad.Describe the process of making a Southern blot.Explain how DNA probes are used to visualize DNA.
Material Type: Lesson
In this lesson the students will:Define Southern blot.Model how Southern blot works using DNA probes that are used to detect specific sequences of DNA.Interpret DNA profiles to determine genetic connections.
Material Type: Activity/Lab
In this lesson the students will:Define polymerase chain reaction (PCR), Taq polymerase, and thermocycler.Describe the stages of PCR.
Material Type: Lesson
In this lesson the students will:Define electropherogram, deoxynucleotides (dNTP), and dideoxynucleotides (ddNTP).Construct a model of a primer for PCR.Construct a model of DNA sequencing using PCR.
Material Type: Lesson
In this lesson the students will:Define the terms forward primer and reverse primer.Demonstrate ability to follow protocols.Conduct PCR to amplify a DNA sequence for future analysis.
Material Type: Activity/Lab
